Everyone, come to look into laboratory cell culture technology to reveal the secret

when it comes to cell culture technology, you may not be unfamiliar with it. It runs through all aspects of cell detection experiments and is the basic factor that determines the success or failure of the experiment. Cell culture refers to a culture technique in which cells are removed from tissues in the body to mimic the environment in the body, under sterile, appropriate temperature, pH and certain nutritional conditions, to grow and reproduce, and to maintain their structure and function. It is used in cytology, Play an important role in the research and application of genetics, virology, and immunology. Cell culture is also a basic technology for virus research and vaccine development. Therefore, cell culture technology has been widely used in genetics, immunology, oncology, virology, molecular biology and other fields. Nuclear transfer, cell hybridization, DNA-mediated gene transfer and the establishment of some physical maps developed in recent years also need to be closely integrated with cell

How many scientific researchers have been frustrated because of the poor state of cell culture! How many laboratories have ever had to terminate their carefully developed long-term experiments due to microbial contamination! So how should cells be cultured to keep them in a normal state? How can we avoid contamination of cultured cells?

Below, Abbkine cell culture method gives you the answer.

The general process of cell culture mainly includes the following points:

  1. Preparatory work: The content of the preparatory work includes the cleaning, drying and disinfection of utensils, the preparation, distribution and sterilization of culture medium and other reagents, the cleaning and disinfection of sterile rooms or ultra-clean benches, and the preparation of incubators and other instruments. Check and debug.
  2. Extracting materials: Take out certain tissue cells from the body in a sterile environment (depending on the purpose of the experiment), and put them into a culture vessel after certain treatments (such as digestion and dispersion of cells, separation, etc.). This process is called extraction . In the case of cell line expansion, there is no such process as obtaining materials. The first culture of tissue cells taken out of the body is called primary culture.
  3. Cultivation: The process of putting the obtained tissue cells into a culture flask or culture plate is called culture. If it is a tissue block culture, connect the tissue block directly to the bottom of the culture vessel. After a few hours, the tissue block can be attached to the bottom, and then add the culture medium.
  4. Cryopreservation and resuscitation: In order to preserve cells, especially mutant cells or cell lines that are not easily available, cells should be frozen. The freezing temperature is generally -196℃ of liquid nitrogen. Collect the cells into the cryopreservation tube and add a medium containing a protective agent (usually dimethyl sulfoxide or glycerol), freeze at a certain cooling rate, and finally save In liquid nitrogen. At extremely low temperatures, the storage time of cells is almost unlimited. The resuscitation generally adopts a quick-thaw method, that is, after removing the cryotube from the liquid nitrogen, immediately put it in 37°C water to make it melt quickly within one minute. Then transfer the cells to a culture vessel for culture.cell culture
  5. The basic steps of cell primary culture:

Primary isolation cell culture refers to the separation of tissues from the donor body into single cells or monotype cell groups by mechanical and digestion, so that they can simulate the physiological environment of the human body in vitro, under sterile, appropriate temperature and certain nutritional conditions. Survive, grow and reproduce. Primary cultured cells often have different cell components and grow slowly, but they are more representative of the tissue cell type from which they are derived and the specific characteristics of the expression tissue. Using primary cell culture to do various experiments, such as drug testing, cell differentiation and virology, has a good effect. The steps are as follows:

  1. Cut the tissue: first clean the obtained tissue with D-Hanks or Hanks solution to remove blood stains on the surface, and use surgical forceps to remove the attached connective tissue and other non-cultivating tissues. After cleaning again, use a scalpel to cut the tissue into several small pieces, transfer them into a penicillin vial or a small beaker, add an appropriate amount of buffer, and use elbow ophthalmic scissors to repeatedly cut the tissue until the tissue becomes a paste, about 1mm3 in size. After standing for a while, use a straw to suck off the upper layer of liquid, add appropriate buffer solution and wash again.
  2. Digestion and separation: 　The purpose of digestion and separation is to digest and separate small tissue pieces into cell clusters or scattered single cells to facilitate further culture. Commonly used digestive enzymes are trypsin and collagenase.
  3. Cultivation: Use a counting plate to count the cells of the cell suspension. Adjust the number of cells to (2～5)×105 cells/ml with the culture medium, or the density required for the experiment, and divide them into culture flasks so that the amount of cell suspension is slightly higher than the bottom of the culture flask after covering. Place in a CO2 incubator, 5% CO2, 37°C static culture. Generally 3~5d, the primary cultured cells can adhere to the wall of the bottle and stretch and start to grow. You can add a new medium of 1/2 of the original medium, continue to culture for 2~3d and then change the medium, generally 7~14d can grow Fill the wall of the bottle and pass it down.

Subculture

Precautions

  1. Digestion time: Master the time for cell digestion. When the digestion time is too short, the cells should not fall off the bottle wall. Excessive digestion will cause the cells to fall off and damage.
  2. Digestion concentration: master the digestion concentration. When the digestion solution concentration is too high, the digestion time should be shortened, and when the digestion solution concentration is too low, the cell digestion time will be relatively prolonged.

Significance of cell cryopreservation

It is a technology that puts cells in a low temperature environment (liquid nitrogen) to reduce cell metabolism for long-term storage. Cryopreservation of cells is one of the main methods of cell preservation, which plays a role in cell preservation.

Significance of cell recovery

Cell resuscitation, a term in biology, refers to the process of re-cultivating cells frozen in liquid nitrogen or in a refrigerator at -70°C to restore growth.

For the great love of “Yinfan”, we will have great scientific research surprises waiting for everyone. At the same time, in order to provide a platform for readers to learn biological knowledge, Abbkine’s technical experts have worked tirelessly to sort out the dry goods of a variety of high-end biological experiments, and later published them in the WeChat public account. Please continue to pay attention to Abbkine for readers who love science the public.

Abbkine focuses on the fields of proteinology and cytology, and is committed to the innovation and research and development of various antibodies, proteins, analytical reagents and kits, in order to become a key promoter in the development of life science research, drug development and other fields. We provide you with the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary antibodies and secondary antibodies for immunological experiments; the favorite products of cell research users, from Dyes and kits for detecting cell status, organelle extraction kits, cell substructure staining and tracking and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help your research career !

when it comes to cell culture technology, you may not be unfamiliar with it. It runs through all aspects of cell detection experiments and is the basic factor that determines the success or failure of the experiment. Cell culture refers to a culture technique in which cells are removed from tissues in the body to mimic the environment in the body, under sterile, appropriate temperature, pH and certain nutritional conditions, to grow and reproduce, and to maintain their structure and function. It is used in cytology, Play an important role in the research and application of genetics, virology, and immunology. Cell culture is also a basic technology for virus research and vaccine development. Therefore, cell culture technology has been widely used in genetics, immunology, oncology, virology, molecular biology and other fields. Nuclear transfer, cell hybridization, DNA-mediated gene transfer and the establishment of some physical maps developed in recent years also need to be closely integrated with cell

How many scientific researchers have been frustrated because of the poor state of cell culture! How many laboratories have ever had to terminate their carefully developed long-term experiments due to microbial contamination! So how should cells be cultured to keep them in a normal state? How can we avoid contamination of cultured cells?

Below, Abbkine cell culture method gives you the answer.

The general process of cell culture mainly includes the following points:

  1. Preparatory work: The content of the preparatory work includes the cleaning, drying and disinfection of utensils, the preparation, distribution and sterilization of culture medium and other reagents, the cleaning and disinfection of sterile rooms or ultra-clean benches, and the preparation of incubators and other instruments. Check and debug.
  2. Extracting materials: Take out certain tissue cells from the body in a sterile environment (depending on the purpose of the experiment), and put them into a culture vessel after certain treatments (such as digestion and dispersion of cells, separation, etc.). This process is called extraction . In the case of cell line expansion, there is no such process as obtaining materials. The first culture of tissue cells taken out of the body is called primary culture.
  3. Cultivation: The process of putting the obtained tissue cells into a culture flask or culture plate is called culture. If it is a tissue block culture, connect the tissue block directly to the bottom of the culture vessel. After a few hours, the tissue block can be attached to the bottom, and then add the culture medium.
  4. Cryopreservation and resuscitation: In order to preserve cells, especially mutant cells or cell lines that are not easily available, cells should be frozen. The freezing temperature is generally -196℃ of liquid nitrogen. Collect the cells into the cryopreservation tube and add a medium containing a protective agent (usually dimethyl sulfoxide or glycerol), freeze at a certain cooling rate, and finally save In liquid nitrogen. At extremely low temperatures, the storage time of cells is almost unlimited. The resuscitation generally adopts a quick-thaw method, that is, after removing the cryotube from the liquid nitrogen, immediately put it in 37°C water to make it melt quickly within one minute. Then transfer the cells to a culture vessel for culture.cell culture
  5. The basic steps of cell primary culture:

Primary isolation cell culture refers to the separation of tissues from the donor body into single cells or monotype cell groups by mechanical and digestion, so that they can simulate the physiological environment of the human body in vitro, under sterile, appropriate temperature and certain nutritional conditions. Survive, grow and reproduce. Primary cultured cells often have different cell components and grow slowly, but they are more representative of the tissue cell type from which they are derived and the specific characteristics of the expression tissue. Using primary cell culture to do various experiments, such as drug testing, cell differentiation and virology, has a good effect. The steps are as follows:

  1. Cut the tissue: first clean the obtained tissue with D-Hanks or Hanks solution to remove blood stains on the surface, and use surgical forceps to remove the attached connective tissue and other non-cultivating tissues. After cleaning again, use a scalpel to cut the tissue into several small pieces, transfer them into a penicillin vial or a small beaker, add an appropriate amount of buffer, and use elbow ophthalmic scissors to repeatedly cut the tissue until the tissue becomes a paste, about 1mm3 in size. After standing for a while, use a straw to suck off the upper layer of liquid, add appropriate buffer solution and wash again.
  2. Digestion and separation: 　The purpose of digestion and separation is to digest and separate small tissue pieces into cell clusters or scattered single cells to facilitate further culture. Commonly used digestive enzymes are trypsin and collagenase.
  3. Cultivation: Use a counting plate to count the cells of the cell suspension. Adjust the number of cells to (2～5)×105 cells/ml with the culture medium, or the density required for the experiment, and divide them into culture flasks so that the amount of cell suspension is slightly higher than the bottom of the culture flask after covering. Place in a CO2 incubator, 5% CO2, 37°C static culture. Generally 3~5d, the primary cultured cells can adhere to the wall of the bottle and stretch and start to grow. You can add a new medium of 1/2 of the original medium, continue to culture for 2~3d and then change the medium, generally 7~14d can grow Fill the wall of the bottle and pass it down.

Subculture

Precautions

  1. Digestion time: Master the time for cell digestion. When the digestion time is too short, the cells should not fall off the bottle wall. Excessive digestion will cause the cells to fall off and damage.
  2. Digestion concentration: master the digestion concentration. When the digestion solution concentration is too high, the digestion time should be shortened, and when the digestion solution concentration is too low, the cell digestion time will be relatively prolonged.

Significance of cell cryopreservation

It is a technology that puts cells in a low temperature environment (liquid nitrogen) to reduce cell metabolism for long-term storage. Cryopreservation of cells is one of the main methods of cell preservation, which plays a role in cell preservation.

Significance of cell recovery

Cell resuscitation, a term in biology, refers to the process of re-cultivating cells frozen in liquid nitrogen or in a refrigerator at -70°C to restore growth.

For the great love of “Yinfan”, we will have great scientific research surprises waiting for everyone. At the same time, in order to provide a platform for readers to learn biological knowledge, Abbkine’s technical experts have worked tirelessly to sort out the dry goods of a variety of high-end biological experiments, and later published them in the WeChat public account. Please continue to pay attention to Abbkine for readers who love science the public.

Abbkine focuses on the fields of proteinology and cytology, and is committed to the innovation and research and development of various antibodies, proteins, analytical reagents and kits, in order to become a key promoter in the development of life science research, drug development and other fields. We provide you with the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary antibodies and secondary antibodies for immunological experiments; the favorite products of cell research users, from Dyes and kits for detecting cell status, organelle extraction kits, cell substructure staining and tracking and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help your research career !

http://www.bioadvisers.com/cell-culture/

Everyone, come to look into laboratory cell culture technology to reveal the secret

when it comes to cell culture technology, you may not be unfamiliar with it. It runs through all aspects of cell detection experiments and is the basic factor that determines the success or failure of the experiment. Cell culture refers to a culture technique in which cells are removed from tissues in the body to mimic the environment in the body, under sterile, appropriate temperature, pH and certain nutritional conditions, to grow and reproduce, and to maintain their structure and function. It is used in cytology, Play an important role in the research and application of genetics, virology, and immunology. Cell culture is also a basic technology for virus research and vaccine development. Therefore, cell culture technology has been widely used in genetics, immunology, oncology, virology, molecular biology and other fields. Nuclear transfer, cell hybridization, DNA-mediated gene transfer and the establishment of some physical maps developed in recent years also need to be closely integrated with cell

How many scientific researchers have been frustrated because of the poor state of cell culture! How many laboratories have ever had to terminate their carefully developed long-term experiments due to microbial contamination! So how should cells be cultured to keep them in a normal state? How can we avoid contamination of cultured cells?

Below, Abbkine cell culture method gives you the answer.

The general process of cell culture mainly includes the following points:

  1. Preparatory work: The content of the preparatory work includes the cleaning, drying and disinfection of utensils, the preparation, distribution and sterilization of culture medium and other reagents, the cleaning and disinfection of sterile rooms or ultra-clean benches, and the preparation of incubators and other instruments. Check and debug.
  2. Extracting materials: Take out certain tissue cells from the body in a sterile environment (depending on the purpose of the experiment), and put them into a culture vessel after certain treatments (such as digestion and dispersion of cells, separation, etc.). This process is called extraction . In the case of cell line expansion, there is no such process as obtaining materials. The first culture of tissue cells taken out of the body is called primary culture.
  3. Cultivation: The process of putting the obtained tissue cells into a culture flask or culture plate is called culture. If it is a tissue block culture, connect the tissue block directly to the bottom of the culture vessel. After a few hours, the tissue block can be attached to the bottom, and then add the culture medium.
  4. Cryopreservation and resuscitation: In order to preserve cells, especially mutant cells or cell lines that are not easily available, cells should be frozen. The freezing temperature is generally -196℃ of liquid nitrogen. Collect the cells into the cryopreservation tube and add a medium containing a protective agent (usually dimethyl sulfoxide or glycerol), freeze at a certain cooling rate, and finally save In liquid nitrogen. At extremely low temperatures, the storage time of cells is almost unlimited. The resuscitation generally adopts a quick-thaw method, that is, after removing the cryotube from the liquid nitrogen, immediately put it in 37°C water to make it melt quickly within one minute. Then transfer the cells to a culture vessel for culture.cell culture
  5. The basic steps of cell primary culture:

Primary isolation cell culture refers to the separation of tissues from the donor body into single cells or monotype cell groups by mechanical and digestion, so that they can simulate the physiological environment of the human body in vitro, under sterile, appropriate temperature and certain nutritional conditions. Survive, grow and reproduce. Primary cultured cells often have different cell components and grow slowly, but they are more representative of the tissue cell type from which they are derived and the specific characteristics of the expression tissue. Using primary cell culture to do various experiments, such as drug testing, cell differentiation and virology, has a good effect. The steps are as follows:

  1. Cut the tissue: first clean the obtained tissue with D-Hanks or Hanks solution to remove blood stains on the surface, and use surgical forceps to remove the attached connective tissue and other non-cultivating tissues. After cleaning again, use a scalpel to cut the tissue into several small pieces, transfer them into a penicillin vial or a small beaker, add an appropriate amount of buffer, and use elbow ophthalmic scissors to repeatedly cut the tissue until the tissue becomes a paste, about 1mm3 in size. After standing for a while, use a straw to suck off the upper layer of liquid, add appropriate buffer solution and wash again.
  2. Digestion and separation: 　The purpose of digestion and separation is to digest and separate small tissue pieces into cell clusters or scattered single cells to facilitate further culture. Commonly used digestive enzymes are trypsin and collagenase.
  3. Cultivation: Use a counting plate to count the cells of the cell suspension. Adjust the number of cells to (2～5)×105 cells/ml with the culture medium, or the density required for the experiment, and divide them into culture flasks so that the amount of cell suspension is slightly higher than the bottom of the culture flask after covering. Place in a CO2 incubator, 5% CO2, 37°C static culture. Generally 3~5d, the primary cultured cells can adhere to the wall of the bottle and stretch and start to grow. You can add a new medium of 1/2 of the original medium, continue to culture for 2~3d and then change the medium, generally 7~14d can grow Fill the wall of the bottle and pass it down.

Subculture

Precautions

  1. Digestion time: Master the time for cell digestion. When the digestion time is too short, the cells should not fall off the bottle wall. Excessive digestion will cause the cells to fall off and damage.
  2. Digestion concentration: master the digestion concentration. When the digestion solution concentration is too high, the digestion time should be shortened, and when the digestion solution concentration is too low, the cell digestion time will be relatively prolonged.

Significance of cell cryopreservation

It is a technology that puts cells in a low temperature environment (liquid nitrogen) to reduce cell metabolism for long-term storage. Cryopreservation of cells is one of the main methods of cell preservation, which plays a role in cell preservation.

Significance of cell recovery

Cell resuscitation, a term in biology, refers to the process of re-cultivating cells frozen in liquid nitrogen or in a refrigerator at -70°C to restore growth.

For the great love of “Yinfan”, we will have great scientific research surprises waiting for everyone. At the same time, in order to provide a platform for readers to learn biological knowledge, Abbkine’s technical experts have worked tirelessly to sort out the dry goods of a variety of high-end biological experiments, and later published them in the WeChat public account. Please continue to pay attention to Abbkine for readers who love science the public.

Abbkine focuses on the fields of proteinology and cytology, and is committed to the innovation and research and development of various antibodies, proteins, analytical reagents and kits, in order to become a key promoter in the development of life science research, drug development and other fields. We provide you with the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary antibodies and secondary antibodies for immunological experiments; the favorite products of cell research users, from Dyes and kits for detecting cell status, organelle extraction kits, cell substructure staining and tracking and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help your research career !

when it comes to cell culture technology, you may not be unfamiliar with it. It runs through all aspects of cell detection experiments and is the basic factor that determines the success or failure of the experiment. Cell culture refers to a culture technique in which cells are removed from tissues in the body to mimic the environment in the body, under sterile, appropriate temperature, pH and certain nutritional conditions, to grow and reproduce, and to maintain their structure and function. It is used in cytology, Play an important role in the research and application of genetics, virology, and immunology. Cell culture is also a basic technology for virus research and vaccine development. Therefore, cell culture technology has been widely used in genetics, immunology, oncology, virology, molecular biology and other fields. Nuclear transfer, cell hybridization, DNA-mediated gene transfer and the establishment of some physical maps developed in recent years also need to be closely integrated with cell

How many scientific researchers have been frustrated because of the poor state of cell culture! How many laboratories have ever had to terminate their carefully developed long-term experiments due to microbial contamination! So how should cells be cultured to keep them in a normal state? How can we avoid contamination of cultured cells?

Below, Abbkine cell culture method gives you the answer.

The general process of cell culture mainly includes the following points:

  1. Preparatory work: The content of the preparatory work includes the cleaning, drying and disinfection of utensils, the preparation, distribution and sterilization of culture medium and other reagents, the cleaning and disinfection of sterile rooms or ultra-clean benches, and the preparation of incubators and other instruments. Check and debug.
  2. Extracting materials: Take out certain tissue cells from the body in a sterile environment (depending on the purpose of the experiment), and put them into a culture vessel after certain treatments (such as digestion and dispersion of cells, separation, etc.). This process is called extraction . In the case of cell line expansion, there is no such process as obtaining materials. The first culture of tissue cells taken out of the body is called primary culture.
  3. Cultivation: The process of putting the obtained tissue cells into a culture flask or culture plate is called culture. If it is a tissue block culture, connect the tissue block directly to the bottom of the culture vessel. After a few hours, the tissue block can be attached to the bottom, and then add the culture medium.
  4. Cryopreservation and resuscitation: In order to preserve cells, especially mutant cells or cell lines that are not easily available, cells should be frozen. The freezing temperature is generally -196℃ of liquid nitrogen. Collect the cells into the cryopreservation tube and add a medium containing a protective agent (usually dimethyl sulfoxide or glycerol), freeze at a certain cooling rate, and finally save In liquid nitrogen. At extremely low temperatures, the storage time of cells is almost unlimited. The resuscitation generally adopts a quick-thaw method, that is, after removing the cryotube from the liquid nitrogen, immediately put it in 37°C water to make it melt quickly within one minute. Then transfer the cells to a culture vessel for culture.cell culture
  5. The basic steps of cell primary culture:

Primary isolation cell culture refers to the separation of tissues from the donor body into single cells or monotype cell groups by mechanical and digestion, so that they can simulate the physiological environment of the human body in vitro, under sterile, appropriate temperature and certain nutritional conditions. Survive, grow and reproduce. Primary cultured cells often have different cell components and grow slowly, but they are more representative of the tissue cell type from which they are derived and the specific characteristics of the expression tissue. Using primary cell culture to do various experiments, such as drug testing, cell differentiation and virology, has a good effect. The steps are as follows:

  1. Cut the tissue: first clean the obtained tissue with D-Hanks or Hanks solution to remove blood stains on the surface, and use surgical forceps to remove the attached connective tissue and other non-cultivating tissues. After cleaning again, use a scalpel to cut the tissue into several small pieces, transfer them into a penicillin vial or a small beaker, add an appropriate amount of buffer, and use elbow ophthalmic scissors to repeatedly cut the tissue until the tissue becomes a paste, about 1mm3 in size. After standing for a while, use a straw to suck off the upper layer of liquid, add appropriate buffer solution and wash again.
  2. Digestion and separation: 　The purpose of digestion and separation is to digest and separate small tissue pieces into cell clusters or scattered single cells to facilitate further culture. Commonly used digestive enzymes are trypsin and collagenase.
  3. Cultivation: Use a counting plate to count the cells of the cell suspension. Adjust the number of cells to (2～5)×105 cells/ml with the culture medium, or the density required for the experiment, and divide them into culture flasks so that the amount of cell suspension is slightly higher than the bottom of the culture flask after covering. Place in a CO2 incubator, 5% CO2, 37°C static culture. Generally 3~5d, the primary cultured cells can adhere to the wall of the bottle and stretch and start to grow. You can add a new medium of 1/2 of the original medium, continue to culture for 2~3d and then change the medium, generally 7~14d can grow Fill the wall of the bottle and pass it down.

Subculture

Precautions

  1. Digestion time: Master the time for cell digestion. When the digestion time is too short, the cells should not fall off the bottle wall. Excessive digestion will cause the cells to fall off and damage.
  2. Digestion concentration: master the digestion concentration. When the digestion solution concentration is too high, the digestion time should be shortened, and when the digestion solution concentration is too low, the cell digestion time will be relatively prolonged.

Significance of cell cryopreservation

It is a technology that puts cells in a low temperature environment (liquid nitrogen) to reduce cell metabolism for long-term storage. Cryopreservation of cells is one of the main methods of cell preservation, which plays a role in cell preservation.

Significance of cell recovery

Cell resuscitation, a term in biology, refers to the process of re-cultivating cells frozen in liquid nitrogen or in a refrigerator at -70°C to restore growth.

For the great love of “Yinfan”, we will have great scientific research surprises waiting for everyone. At the same time, in order to provide a platform for readers to learn biological knowledge, Abbkine’s technical experts have worked tirelessly to sort out the dry goods of a variety of high-end biological experiments, and later published them in the WeChat public account. Please continue to pay attention to Abbkine for readers who love science the public.

Abbkine focuses on the fields of proteinology and cytology, and is committed to the innovation and research and development of various antibodies, proteins, analytical reagents and kits, in order to become a key promoter in the development of life science research, drug development and other fields. We provide you with the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary antibodies and secondary antibodies for immunological experiments; the favorite products of cell research users, from Dyes and kits for detecting cell status, organelle extraction kits, cell substructure staining and tracking and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help your research career !

http://www.bioadvisers.com/cell-culture/

Abbkine LDH test kit, we want to say…

 

The last issue of Abbkine’s WeChat public account article tells about an important indicator of cell state detection, witnessing the state of cell viability. This is also the “good news” for many cytology researchers. Once the article was published, the response was very good, and everyone asked questions:

A: The Cell Living Dead Kit is so easy to use, we also want to start it. What is its purchase link?

B: What is the quotation for this kit? Is it family price?

C: Abbkine’s Living Dead Staining Kit is so easy to use, is there any other kit that can be used together?

D: In our laboratory, we do cell toxicology experiments. In addition to testing the effects of drugs on cell viability, can we also detect other states of cells?

For product purchase links and price questions, please leave a message below the article, and Abbkine’s beautiful little brothers and sisters will enthusiastically answer for everyone. For the combination of products, Abbkine, in order to help you detect more cell status indicators and more comprehensively evaluate the impact of external stimuli on cells, we have launched the LDH kit cytotoxicity detection kit (KTA1030).

Product NO. Product Name Product Links
KTA1030 LDH Cytotoxicity Assay Kit https://www.abbkine.com/product/ldh-cytotoxicity-assay-kit-kta1030/

Popular science: lactate dehydrogenase (LDH or LD) is an important enzyme that catalyzes the redox reaction between lactic acid and pyruvate in glycolysis and gluconeogenesis engineering. Lactate dehydrogenase exists in the cytoplasm of all tissues and cells of the body, among which the content of the kidney is higher. In terms of the rate of glycolysis, lactate dehydrogenase is not a rate-limiting enzyme, so it has little effect on the rate of occurrence. Using this material, we quantitatively evaluate cell death or cytotoxicity through plasma membrane damage. LDH is a stable enzyme that exists in all cell types and is quickly released into the cell culture medium after the plasma membrane is damaged. Therefore, LDH is the most widely used marker in cytotoxicity studies.

The application range is very wide:

Analyze the effects of compounds or environmental factors on cell death. All animal cell samples can be tested. Detection range of cell number: 10,000-100,0000 cells/well.

Easy to operate:

  1. No need to draw a standard curve, use positive and negative results to quantify cytotoxicity;
  2. LDH is released from the cells into the medium, forming a visual color response. After the sample reaction is colored, use a microplate reader for detection, and the reading is convenient;
  3. Samples and reaction equipment are easily available: only 200 μL of cell culture supernatant is required as a sample for subsequent testing. Ordinary 96-well plates can be completed.

This issue mainly talks about the experimental protocols and products of cytotoxicity detection in cytology research. Due to space limitations, I have to say goodbye to everyone again. At the same time in the next issue, there will be experiments and product application operation tips to meet with you. Stay tuned~~~~~

You are welcome to pay more attention to Abbkine’s official account, and there will be more scientific research surprises waiting for you. At the same time, in order to provide a platform for readers to learn biological knowledge, Abbkine’s technical experts have worked tirelessly to sort out the dry goods of a variety of high-end biological experiments, and later published them in the WeChat public account. Please continue to pay attention to Abbkine for readers who love science. the public.

Abbkine focuses on the fields of proteinology and cytology, and is committed to the innovation and research and development of various antibodies, proteins, analytical reagents and kits, in order to become a key promoter in the development of life science research, drug development and other fields. We provide you with the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary antibodies and secondary antibodies for immunological experiments; the favorite products of cell research users, from Dyes and kits for detecting cell status, organelle extraction kits, cell substructure staining and tracking and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help your research career

 

 

The last issue of Abbkine’s WeChat public account article tells about an important indicator of cell state detection, witnessing the state of cell viability. This is also the “good news” for many cytology researchers. Once the article was published, the response was very good, and everyone asked questions:

A: The Cell Living Dead Kit is so easy to use, we also want to start it. What is its purchase link?

B: What is the quotation for this kit? Is it family price?

C: Abbkine’s Living Dead Staining Kit is so easy to use, is there any other kit that can be used together?

D: In our laboratory, we do cell toxicology experiments. In addition to testing the effects of drugs on cell viability, can we also detect other states of cells?

For product purchase links and price questions, please leave a message below the article, and Abbkine’s beautiful little brothers and sisters will enthusiastically answer for everyone. For the combination of products, Abbkine, in order to help you detect more cell status indicators and more comprehensively evaluate the impact of external stimuli on cells, we have launched the LDH kit cytotoxicity detection kit (KTA1030).

Product NO. Product Name Product Links
KTA1030 LDH Cytotoxicity Assay Kit https://www.abbkine.com/product/ldh-cytotoxicity-assay-kit-kta1030/

Popular science: lactate dehydrogenase (LDH or LD) is an important enzyme that catalyzes the redox reaction between lactic acid and pyruvate in glycolysis and gluconeogenesis engineering. Lactate dehydrogenase exists in the cytoplasm of all tissues and cells of the body, among which the content of the kidney is higher. In terms of the rate of glycolysis, lactate dehydrogenase is not a rate-limiting enzyme, so it has little effect on the rate of occurrence. Using this material, we quantitatively evaluate cell death or cytotoxicity through plasma membrane damage. LDH is a stable enzyme that exists in all cell types and is quickly released into the cell culture medium after the plasma membrane is damaged. Therefore, LDH is the most widely used marker in cytotoxicity studies.

The application range is very wide:

Analyze the effects of compounds or environmental factors on cell death. All animal cell samples can be tested. Detection range of cell number: 10,000-100,0000 cells/well.

Easy to operate:

  1. No need to draw a standard curve, use positive and negative results to quantify cytotoxicity;
  2. LDH is released from the cells into the medium, forming a visual color response. After the sample reaction is colored, use a microplate reader for detection, and the reading is convenient;
  3. Samples and reaction equipment are easily available: only 200 μL of cell culture supernatant is required as a sample for subsequent testing. Ordinary 96-well plates can be completed.

This issue mainly talks about the experimental protocols and products of cytotoxicity detection in cytology research. Due to space limitations, I have to say goodbye to everyone again. At the same time in the next issue, there will be experiments and product application operation tips to meet with you. Stay tuned~~~~~

You are welcome to pay more attention to Abbkine’s official account, and there will be more scientific research surprises waiting for you. At the same time, in order to provide a platform for readers to learn biological knowledge, Abbkine’s technical experts have worked tirelessly to sort out the dry goods of a variety of high-end biological experiments, and later published them in the WeChat public account. Please continue to pay attention to Abbkine for readers who love science. the public.

Abbkine focuses on the fields of proteinology and cytology, and is committed to the innovation and research and development of various antibodies, proteins, analytical reagents and kits, in order to become a key promoter in the development of life science research, drug development and other fields. We provide you with the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary antibodies and secondary antibodies for immunological experiments; the favorite products of cell research users, from Dyes and kits for detecting cell status, organelle extraction kits, cell substructure staining and tracking and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help your research career

 

http://www.bioadvisers.com/cytotoxicity/

How to comprehensively study cell proliferation, the Abbkine EdU test kit turns a stone into gold

 

Cell proliferation is an important life characteristic of organisms, and cells proliferate by dividing. Single-celled organisms that produce new individuals through cell division. Multicellular organisms produce new cells through cell division to supplement aging or dead cells in the body. Multicellular organisms can develop into a new multicellular individual from a fertilized egg through cell division and differentiation. It must be emphasized that through cell division, the replicated genetic material can be evenly distributed to two daughter cells. It can be seen that cell proliferation is the basis of organism growth, development, reproduction and heredity.

It can be said that cell proliferation is accompanied by all the life processes of multicellular organisms. More and more researchers have focused on cell proliferation detection, and the methods for cell proliferation detection are constantly updated. So what are the methods to study cell proliferation so far?

EdU

Cell proliferation testing is a basic experimental method to evaluate cell health, genotoxicity and the effect of anti-tumor drugs. The most accurate method for detecting cell proliferation is the BrdU method. The EdU method detection kit is a revolutionary breakthrough in the Brdu method. EdU (5-bromo-2-deoxyuracil) kit is a pyrimidine analog that can be integrated into DNA double strands during DNA synthesis. Through the comparison of the above schematic diagrams, it is not difficult to find that the EdU method is a simple, reliable, and trouble-free innovative method for detecting cell proliferation without antibodies, no denaturation steps, and maintaining cell morphology and DNA integrity.

However, there are still many researchers who still do not get the most effective method for cell proliferation testing and save reaction time. At this time, Abbkine’s EdU test kit came into being with the joint efforts of R&D experts, which solved everyone’s urgent need. And Abbkine’s EdU detection kit has two models, which are matched with two different fluorescent channels to meet the needs of different customers for fluorescent channels. Increase the diversity of cell proliferation test results.

Product NO. Product NO. Fluorescence channel
KTA2030 Cell Proliferation EdU Image Kit (Green Fluorescence) 488 channel
KTA2031 Cell Proliferation EdU Image Kit (Orange Fluorescence) 549 channel

Moreover, the kit can not only label proliferating cells, but also use nuclear dyes to label the nuclei of all cells (including proliferating cells and non-proliferating cells). Proliferating cells can be obtained by the number of proliferating cells and the number of all cells. The ratio. The calculation result is accurate and reliable, and the application value is extremely wide. Here are some pictures of the test results of the Abbkine EdU test kit:

DAPI Channel

DAPI  Channel

549 Channel

549 Channel

488 Channel

488 Channel

This issue mainly talks about the overall solution for cytology research. I have to say goodbye to everyone again. At the same time in the next issue, there will be experiments and product application operation tips to meet with you. Stay tuned~~~~~

 

You are welcome to pay more attention to Abbkine’s official account, and there will be more scientific research surprises waiting for everyone. At the same time, in order to provide readers with a platform for learning biological knowledge, Abbkine’s technical experts have worked tirelessly to sort out the dry goods of a variety of high-end biological experiments, which will be published in the WeChat public account in the future. Please continue to pay attention to Abbkine for readers who love science. the public.

Abbkine focuses on the fields of proteinology and cytology, and is committed to the innovation and research and development of various antibodies, proteins, analytical reagents and kits, in order to become a key promoter in the development of life science research, drug development and other fields. We provide you with the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary antibodies and secondary antibodies for immunological experiments; the favorite products of cell research users, from Dyes and kits for detecting cell status, organelle extraction kits, cell substructure staining and tracking and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help your research career !

 

 

Cell proliferation is an important life characteristic of organisms, and cells proliferate by dividing. Single-celled organisms that produce new individuals through cell division. Multicellular organisms produce new cells through cell division to supplement aging or dead cells in the body. Multicellular organisms can develop into a new multicellular individual from a fertilized egg through cell division and differentiation. It must be emphasized that through cell division, the replicated genetic material can be evenly distributed to two daughter cells. It can be seen that cell proliferation is the basis of organism growth, development, reproduction and heredity.

It can be said that cell proliferation is accompanied by all the life processes of multicellular organisms. More and more researchers have focused on cell proliferation detection, and the methods for cell proliferation detection are constantly updated. So what are the methods to study cell proliferation so far?

EdU

Cell proliferation testing is a basic experimental method to evaluate cell health, genotoxicity and the effect of anti-tumor drugs. The most accurate method for detecting cell proliferation is the BrdU method. The EdU method detection kit is a revolutionary breakthrough in the Brdu method. EdU (5-bromo-2-deoxyuracil) kit is a pyrimidine analog that can be integrated into DNA double strands during DNA synthesis. Through the comparison of the above schematic diagrams, it is not difficult to find that the EdU method is a simple, reliable, and trouble-free innovative method for detecting cell proliferation without antibodies, no denaturation steps, and maintaining cell morphology and DNA integrity.

However, there are still many researchers who still do not get the most effective method for cell proliferation testing and save reaction time. At this time, Abbkine’s EdU test kit came into being with the joint efforts of R&D experts, which solved everyone’s urgent need. And Abbkine’s EdU detection kit has two models, which are matched with two different fluorescent channels to meet the needs of different customers for fluorescent channels. Increase the diversity of cell proliferation test results.

Product NO. Product NO. Fluorescence channel
KTA2030 Cell Proliferation EdU Image Kit (Green Fluorescence) 488 channel
KTA2031 Cell Proliferation EdU Image Kit (Orange Fluorescence) 549 channel

Moreover, the kit can not only label proliferating cells, but also use nuclear dyes to label the nuclei of all cells (including proliferating cells and non-proliferating cells). Proliferating cells can be obtained by the number of proliferating cells and the number of all cells. The ratio. The calculation result is accurate and reliable, and the application value is extremely wide. Here are some pictures of the test results of the Abbkine EdU test kit:

DAPI Channel

DAPI  Channel

549 Channel

549 Channel

488 Channel

488 Channel

This issue mainly talks about the overall solution for cytology research. I have to say goodbye to everyone again. At the same time in the next issue, there will be experiments and product application operation tips to meet with you. Stay tuned~~~~~

 

You are welcome to pay more attention to Abbkine’s official account, and there will be more scientific research surprises waiting for everyone. At the same time, in order to provide readers with a platform for learning biological knowledge, Abbkine’s technical experts have worked tirelessly to sort out the dry goods of a variety of high-end biological experiments, which will be published in the WeChat public account in the future. Please continue to pay attention to Abbkine for readers who love science. the public.

Abbkine focuses on the fields of proteinology and cytology, and is committed to the innovation and research and development of various antibodies, proteins, analytical reagents and kits, in order to become a key promoter in the development of life science research, drug development and other fields. We provide you with the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary antibodies and secondary antibodies for immunological experiments; the favorite products of cell research users, from Dyes and kits for detecting cell status, organelle extraction kits, cell substructure staining and tracking and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help your research career !

 

http://www.bioadvisers.com/comprehensively-study-cell-proliferation-abbkine-edu-test-kit-turns-stone-gold/

How to choose flow cytometry antibody?

Flow cytometry is a very practical technique. It is characterized by convenience and quickness, accurate results, and successful sorting of target cells. However, when it comes to the specific selection of flow cytometry antibodies, it is estimated that many small partners will have difficulties. It is not so easy to obtain a beautiful flow cytometry result graph. The most important thing to consider before the experiment is the choice of antibody and the combination of fluorescein.

What issues should be paid attention to when selecting flow cytometry antibodies?

1.Basic requirements for antibodies

a.Know clearly whether the target to be tested is intracellular or extracellular protein;

b.Select antibody reactivity in strict accordance with the source of the sample;

c.You must choose antibodies that are clearly labeled for use in flow cytometry experiments and cannot be replaced by ordinary antibodies.

2.Requirements for flow cytometry

a.Understand that the flow cytometer used has several lasers, the common lasers are 405nm, 488nm and 633nm, etc. At present, most flow cytometers are equipped with 2 lasers of 488nm and 633nm;

b.Know how many detection channels are under each laser, and the number of channels determines how many indicators can be measured at a time;

Instrument Common Excitation （Ex） light Fluorescence channel Common fluorescent label
flow cytometry 488nm 530/30 FITC, DyLight 488, Abfluor 488
575/26 PE
610/20 PE/TR
695/40 PerCP/Cyanine5.5、 PE/Cyanine5、PerCP
780/60 PE/Cyanine7
633nm 660/20 APC、 Abfluor 647
730/45 Abfluor 750
780/60 APC/Cyanine7

3.Choice of fluorescent label

a.If there is a direct-labeled antibody, try to choose the direct-labeled antibody as much as possible. Indirect labeling of the secondary antibody increases the experimental steps. Multiple washings will cause cell loss and low staining accuracy;

b.Weakly express the target, try to choose a strong fluorescein-labeled antibody, PE> APC> FITC> PerCP.

4.Multi-color matching scheme

a.Each channel can only use one type of label, such as FITC and AF488. The excitation and emission wavelengths of the two labels are similar, so these two labels cannot be used at the same time;

b.Minimize spectral overlap, such as PE-cy5 and APC, adjust the compensation value to be too large when both are used;

c.Weak targets are equipped with strong fluorescein. For example, the expression of CD4 in Treg cells is higher than FoxP3, so FoxP3 should use strong fluorescein-labeled antibodies;

d.It is recommended to consult the literature for five-color and above color matching, or consult the antibody manufacturer.

5.How to adjust compensation?

In multicolor flow experiments, due to the overlap of the spectra of each fluorescein, compensation adjustment is required. The following figure shows the spectra of FITC and PE fluoresceins. Near 550nm, the two dyes have spectral overlap.

6.Isotype control?

The purpose of isotype control is to reduce background interference and better display the binding strength of antigen and antibody. Especially for some intracellular proteins, low expression or continuous expression proteins, the setting of isotype control is particularly necessary. Isotype control needs to select antibodies with the same species source, the same antibody subtype, and the same fluorescent label as the stained monoclonal antibody.

Recommendation: Elabscience flow cytometry antibody one-a more cost-effective overall solution for flow cytometry experiments

Elabscience has selected clone numbers reported many times in the literature as the source of flow cytometry antibodies, anti-human/rat/mouse, etc., and has a wealth of indicators, with> 1480 online indicators covering most common flow cytometry antibodies, and There are 12 kinds of fluorescent labels, which can meet the needs of many kinds of experiments. In addition, all fluorescent antibodies provide convenient Test specifications for your direct use. Most importantly, Elabscience can also customize the flow color scheme for free, so you don’t have to worry about multicolor experiments.

Product example image:

compensation

Figure six indicators are detected simultaneously, the negative and positive cell populations of all indicators can be fully separated.

About Abbkine

Our position: to serve global users of cell and protein research, to provide users with economical and technical product solutions in the form of application processes and product portfolios.

Our mission: to stimulate our inner creativity, provide competitive biomedical products and services, and continue to create maximum value for customers.

Our vision: to be a respected, world-class supplier of biomedical products and services.

 

Flow cytometry is a very practical technique. It is characterized by convenience and quickness, accurate results, and successful sorting of target cells. However, when it comes to the specific selection of flow cytometry antibodies, it is estimated that many small partners will have difficulties. It is not so easy to obtain a beautiful flow cytometry result graph. The most important thing to consider before the experiment is the choice of antibody and the combination of fluorescein.

What issues should be paid attention to when selecting flow cytometry antibodies?

1.Basic requirements for antibodies

a.Know clearly whether the target to be tested is intracellular or extracellular protein;

b.Select antibody reactivity in strict accordance with the source of the sample;

c.You must choose antibodies that are clearly labeled for use in flow cytometry experiments and cannot be replaced by ordinary antibodies.

2.Requirements for flow cytometry

a.Understand that the flow cytometer used has several lasers, the common lasers are 405nm, 488nm and 633nm, etc. At present, most flow cytometers are equipped with 2 lasers of 488nm and 633nm;

b.Know how many detection channels are under each laser, and the number of channels determines how many indicators can be measured at a time;

Instrument Common Excitation （Ex） light Fluorescence channel Common fluorescent label
flow cytometry 488nm 530/30 FITC, DyLight 488, Abfluor 488
575/26 PE
610/20 PE/TR
695/40 PerCP/Cyanine5.5、 PE/Cyanine5、PerCP
780/60 PE/Cyanine7
633nm 660/20 APC、 Abfluor 647
730/45 Abfluor 750
780/60 APC/Cyanine7

3.Choice of fluorescent label

a.If there is a direct-labeled antibody, try to choose the direct-labeled antibody as much as possible. Indirect labeling of the secondary antibody increases the experimental steps. Multiple washings will cause cell loss and low staining accuracy;

b.Weakly express the target, try to choose a strong fluorescein-labeled antibody, PE> APC> FITC> PerCP.

4.Multi-color matching scheme

a.Each channel can only use one type of label, such as FITC and AF488. The excitation and emission wavelengths of the two labels are similar, so these two labels cannot be used at the same time;

b.Minimize spectral overlap, such as PE-cy5 and APC, adjust the compensation value to be too large when both are used;

c.Weak targets are equipped with strong fluorescein. For example, the expression of CD4 in Treg cells is higher than FoxP3, so FoxP3 should use strong fluorescein-labeled antibodies;

d.It is recommended to consult the literature for five-color and above color matching, or consult the antibody manufacturer.

5.How to adjust compensation?

In multicolor flow experiments, due to the overlap of the spectra of each fluorescein, compensation adjustment is required. The following figure shows the spectra of FITC and PE fluoresceins. Near 550nm, the two dyes have spectral overlap.

6.Isotype control?

The purpose of isotype control is to reduce background interference and better display the binding strength of antigen and antibody. Especially for some intracellular proteins, low expression or continuous expression proteins, the setting of isotype control is particularly necessary. Isotype control needs to select antibodies with the same species source, the same antibody subtype, and the same fluorescent label as the stained monoclonal antibody.

Recommendation: Elabscience flow cytometry antibody one-a more cost-effective overall solution for flow cytometry experiments

Elabscience has selected clone numbers reported many times in the literature as the source of flow cytometry antibodies, anti-human/rat/mouse, etc., and has a wealth of indicators, with> 1480 online indicators covering most common flow cytometry antibodies, and There are 12 kinds of fluorescent labels, which can meet the needs of many kinds of experiments. In addition, all fluorescent antibodies provide convenient Test specifications for your direct use. Most importantly, Elabscience can also customize the flow color scheme for free, so you don’t have to worry about multicolor experiments.

Product example image:

compensation

Figure six indicators are detected simultaneously, the negative and positive cell populations of all indicators can be fully separated.

About Abbkine

Our position: to serve global users of cell and protein research, to provide users with economical and technical product solutions in the form of application processes and product portfolios.

Our mission: to stimulate our inner creativity, provide competitive biomedical products and services, and continue to create maximum value for customers.

Our vision: to be a respected, world-class supplier of biomedical products and services.

 

http://www.bioadvisers.com/t-cells-2/

High Uric Acid Level and Gout

 

Uric acid is a natural waste product from the digestion of foods that contain purines. Purines are normally produced in the body and are also found in some foods and drinks. Foods with high content of purines include liver, anchovies, mackerel, dried beans and peas, and beer.

 

Gout occurs when urate crystals accumulate in your joint, causing the inflammation and intense pain of a gout attack. Urate crystals can form when you have high levels of uric acid in your blood.

Your body produces uric acid when it breaks down purines — substances that are found naturally in your body.

 

Fig 1: Metabolic pathways of uric acid formation from nucleotide monophosphates.

(Picture Source: https://pubmed.ncbi.nlm.nih.gov/24287461/ )

 

Through year of efforts, Abbkine research scientists have developed a series of assay kits for cell metabolism and that constitutes Abbkine CheKine™group. For detailed index, you are welcome to view at https://www.abbkine.com/?s=CheKine%E2%84%A2&post_type=product&submit=. In which we can see the detection kits of NAD/NADH, NADP/NADPH, superoxide dismutases (SOD), peroxidase (HRP) and glucose, but today we are going to introduce the test of uric acid for you.

 

To more specific, the benefits of Abbkine Uric Acid (UA) Colorimetric Assay Kit can be concluded as below:

  • Determination of Uric Acid (UA) concentration in a variety of biological samples such as animal tissues, serum,urine and other biological fluids.
  • Detailed sample preparation and result calculation methods are provided.
  • Simple operation steps.

You can view the kit protocol at https://www.abbkine.com/file/booklet/KTB1510-B.pdf

 

Kit Components:

  • Extraction Buffer
  • Reagent I A
  • Reagent I B
  • Standard

 

Abbkine is aimed at to provide high quality and cost effective reagents to global scientists, this    Uric Acid (UA) Colorimetric Assay Kit has been optimized to use and can deduct your experiment cost. For detailed product information, you can refer in below sheet.

CAT# Product Name Size and Price
KTB1510 CheKine™ Uric Acid (UA) Colorimetric Assay Kit $90/48T

$160/96T

If you are interested in Abbkine CheKine™Uric Acid (UA) Colorimetric Assay Kit, feel free to contact service@abbkine.com, our service representative will get back to you shortly!

 

About Abbkine Scientific Co., Ltd.

Abbkine offers services for global scientists in the fields of proteomics and cytology, and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, to promote the development of fields including life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, to help your research career!

 

 

Uric acid is a natural waste product from the digestion of foods that contain purines. Purines are normally produced in the body and are also found in some foods and drinks. Foods with high content of purines include liver, anchovies, mackerel, dried beans and peas, and beer.

 

Gout occurs when urate crystals accumulate in your joint, causing the inflammation and intense pain of a gout attack. Urate crystals can form when you have high levels of uric acid in your blood.

Your body produces uric acid when it breaks down purines — substances that are found naturally in your body.

 

Fig 1: Metabolic pathways of uric acid formation from nucleotide monophosphates.

(Picture Source: https://pubmed.ncbi.nlm.nih.gov/24287461/ )

 

Through year of efforts, Abbkine research scientists have developed a series of assay kits for cell metabolism and that constitutes Abbkine CheKine™group. For detailed index, you are welcome to view at https://www.abbkine.com/?s=CheKine%E2%84%A2&post_type=product&submit=. In which we can see the detection kits of NAD/NADH, NADP/NADPH, superoxide dismutases (SOD), peroxidase (HRP) and glucose, but today we are going to introduce the test of uric acid for you.

 

To more specific, the benefits of Abbkine Uric Acid (UA) Colorimetric Assay Kit can be concluded as below:

  • Determination of Uric Acid (UA) concentration in a variety of biological samples such as animal tissues, serum,urine and other biological fluids.
  • Detailed sample preparation and result calculation methods are provided.
  • Simple operation steps.

You can view the kit protocol at https://www.abbkine.com/file/booklet/KTB1510-B.pdf

 

Kit Components:

  • Extraction Buffer
  • Reagent I A
  • Reagent I B
  • Standard

 

Abbkine is aimed at to provide high quality and cost effective reagents to global scientists, this    Uric Acid (UA) Colorimetric Assay Kit has been optimized to use and can deduct your experiment cost. For detailed product information, you can refer in below sheet.

CAT# Product Name Size and Price
KTB1510 CheKine™ Uric Acid (UA) Colorimetric Assay Kit $90/48T

$160/96T

If you are interested in Abbkine CheKine™Uric Acid (UA) Colorimetric Assay Kit, feel free to contact service@abbkine.com, our service representative will get back to you shortly!

 

About Abbkine Scientific Co., Ltd.

Abbkine offers services for global scientists in the fields of proteomics and cytology, and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, to promote the development of fields including life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, to help your research career!

 

http://www.bioadvisers.com/purine/

High Uric Acid Level and Gout

 

Uric acid is a natural waste product from the digestion of foods that contain purines. Purines are normally produced in the body and are also found in some foods and drinks. Foods with high content of purines include liver, anchovies, mackerel, dried beans and peas, and beer.

 

Gout occurs when urate crystals accumulate in your joint, causing the inflammation and intense pain of a gout attack. Urate crystals can form when you have high levels of uric acid in your blood.

Your body produces uric acid when it breaks down purines — substances that are found naturally in your body.

 

Fig 1: Metabolic pathways of uric acid formation from nucleotide monophosphates.

(Picture Source: https://pubmed.ncbi.nlm.nih.gov/24287461/ )

 

Through year of efforts, Abbkine research scientists have developed a series of assay kits for cell metabolism and that constitutes Abbkine CheKine™group. For detailed index, you are welcome to view at https://www.abbkine.com/?s=CheKine%E2%84%A2&post_type=product&submit=. In which we can see the detection kits of NAD/NADH, NADP/NADPH, superoxide dismutases (SOD), peroxidase (HRP) and glucose, but today we are going to introduce the test of uric acid for you.

 

To more specific, the benefits of Abbkine Uric Acid (UA) Colorimetric Assay Kit can be concluded as below:

  • Determination of Uric Acid (UA) concentration in a variety of biological samples such as animal tissues, serum,urine and other biological fluids.
  • Detailed sample preparation and result calculation methods are provided.
  • Simple operation steps.

You can view the kit protocol at https://www.abbkine.com/file/booklet/KTB1510-B.pdf

 

Kit Components:

  • Extraction Buffer
  • Reagent I A
  • Reagent I B
  • Standard

 

Abbkine is aimed at to provide high quality and cost effective reagents to global scientists, this    Uric Acid (UA) Colorimetric Assay Kit has been optimized to use and can deduct your experiment cost. For detailed product information, you can refer in below sheet.

CAT# Product Name Size and Price
KTB1510 CheKine™ Uric Acid (UA) Colorimetric Assay Kit $90/48T

$160/96T

If you are interested in Abbkine CheKine™Uric Acid (UA) Colorimetric Assay Kit, feel free to contact service@abbkine.com, our service representative will get back to you shortly!

 

About Abbkine Scientific Co., Ltd.

Abbkine offers services for global scientists in the fields of proteomics and cytology, and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, to promote the development of fields including life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, to help your research career!

 

 

Uric acid is a natural waste product from the digestion of foods that contain purines. Purines are normally produced in the body and are also found in some foods and drinks. Foods with high content of purines include liver, anchovies, mackerel, dried beans and peas, and beer.

 

Gout occurs when urate crystals accumulate in your joint, causing the inflammation and intense pain of a gout attack. Urate crystals can form when you have high levels of uric acid in your blood.

Your body produces uric acid when it breaks down purines — substances that are found naturally in your body.

 

Fig 1: Metabolic pathways of uric acid formation from nucleotide monophosphates.

(Picture Source: https://pubmed.ncbi.nlm.nih.gov/24287461/ )

 

Through year of efforts, Abbkine research scientists have developed a series of assay kits for cell metabolism and that constitutes Abbkine CheKine™group. For detailed index, you are welcome to view at https://www.abbkine.com/?s=CheKine%E2%84%A2&post_type=product&submit=. In which we can see the detection kits of NAD/NADH, NADP/NADPH, superoxide dismutases (SOD), peroxidase (HRP) and glucose, but today we are going to introduce the test of uric acid for you.

 

To more specific, the benefits of Abbkine Uric Acid (UA) Colorimetric Assay Kit can be concluded as below:

  • Determination of Uric Acid (UA) concentration in a variety of biological samples such as animal tissues, serum,urine and other biological fluids.
  • Detailed sample preparation and result calculation methods are provided.
  • Simple operation steps.

You can view the kit protocol at https://www.abbkine.com/file/booklet/KTB1510-B.pdf

 

Kit Components:

  • Extraction Buffer
  • Reagent I A
  • Reagent I B
  • Standard

 

Abbkine is aimed at to provide high quality and cost effective reagents to global scientists, this    Uric Acid (UA) Colorimetric Assay Kit has been optimized to use and can deduct your experiment cost. For detailed product information, you can refer in below sheet.

CAT# Product Name Size and Price
KTB1510 CheKine™ Uric Acid (UA) Colorimetric Assay Kit $90/48T

$160/96T

If you are interested in Abbkine CheKine™Uric Acid (UA) Colorimetric Assay Kit, feel free to contact service@abbkine.com, our service representative will get back to you shortly!

 

About Abbkine Scientific Co., Ltd.

Abbkine offers services for global scientists in the fields of proteomics and cytology, and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, to promote the development of fields including life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, to help your research career!

 

http://www.bioadvisers.com/purine/

New Product Arrival, ALT and AST Test Kit Available from Abbkine!

Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are enzymes found mainly in the liver, but also found in red blood cells, heart cells, muscle tissue and other organs, such as the pancreas and kidneys. An AST test is often performed along with an alanine aminotransferase (ALT) test. Both are enzymes found in the liver that become elevated in the blood when the liver is damaged.

 

AST is usually done as part of a group of liver function tests called a liver panel. It’s often ordered with a test for alanine aminotransferase (ALT), another liver enzyme. ALT is more accurate than AST at detecting liver disease. Abbkine has newly launched ALT test kit to help scientific research.

TCA Tycle

Fig 1: TCA Tycle

(Picture Source: https://www.mdpi.com/1422-0067/20/2/252)

 

Through year of efforts, Abbkine research scientists have developed a series of assay kits for cell metabolism and that constitutes Abbkine CheKine™group. In which we can see the detection kits of NAD/NADH, NADP/NADPH, superoxide dismutases (SOD), peroxidase (HRP) and glucose, but today we are going to introduce the test of ALT for you.

 

To more specific, the benefits of Abbkine Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit can be concluded as below:

  • Determination of ALT/GPT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial.
  • Detailed sample preparation and result calculation methods are provided.
  • Simple operation steps.
  • Standard and standard curve line are provided.

 

You can view the kit protocol at https://www.abbkine.com/file/booklet/KTB1410-B.pdf .

 

Kit Components:

  • Extraction Buffer
  • Reagent I
  • Reagent II
  • Reagent III
  • Standard

 

Abbkine is aimed at to provide high quality and cost effective reagents to global scientists, this    Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit has been optimized to use and can deduct your experiment cost. For detailed product information, you can refer in below sheet.

 

CAT# Product Name Size and Price
KTB1410 CheKine™ Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit $40/96T

 

If you are interested in Abbkine Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit, feel free to contact service@abbkine.com, our service representative will get back to you shortly!

 

About Abbkine Scientific Co., Ltd.

Abbkine offers services for global scientists in the fields of proteomics and cytology, and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, to promote the development of fields including life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, to help your research career!

 

Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are enzymes found mainly in the liver, but also found in red blood cells, heart cells, muscle tissue and other organs, such as the pancreas and kidneys. An AST test is often performed along with an alanine aminotransferase (ALT) test. Both are enzymes found in the liver that become elevated in the blood when the liver is damaged.

 

AST is usually done as part of a group of liver function tests called a liver panel. It’s often ordered with a test for alanine aminotransferase (ALT), another liver enzyme. ALT is more accurate than AST at detecting liver disease. Abbkine has newly launched ALT test kit to help scientific research.

TCA Tycle

Fig 1: TCA Tycle

(Picture Source: https://www.mdpi.com/1422-0067/20/2/252)

 

Through year of efforts, Abbkine research scientists have developed a series of assay kits for cell metabolism and that constitutes Abbkine CheKine™group. In which we can see the detection kits of NAD/NADH, NADP/NADPH, superoxide dismutases (SOD), peroxidase (HRP) and glucose, but today we are going to introduce the test of ALT for you.

 

To more specific, the benefits of Abbkine Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit can be concluded as below:

  • Determination of ALT/GPT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial.
  • Detailed sample preparation and result calculation methods are provided.
  • Simple operation steps.
  • Standard and standard curve line are provided.

 

You can view the kit protocol at https://www.abbkine.com/file/booklet/KTB1410-B.pdf .

 

Kit Components:

  • Extraction Buffer
  • Reagent I
  • Reagent II
  • Reagent III
  • Standard

 

Abbkine is aimed at to provide high quality and cost effective reagents to global scientists, this    Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit has been optimized to use and can deduct your experiment cost. For detailed product information, you can refer in below sheet.

 

CAT# Product Name Size and Price
KTB1410 CheKine™ Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit $40/96T

 

If you are interested in Abbkine Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit, feel free to contact service@abbkine.com, our service representative will get back to you shortly!

 

About Abbkine Scientific Co., Ltd.

Abbkine offers services for global scientists in the fields of proteomics and cytology, and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, to promote the development of fields including life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, to help your research career!

 

http://www.bioadvisers.com/alanine-aminotransferase/

New Product Arrival, ALT and AST Test Kit Available from Abbkine!

Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are enzymes found mainly in the liver, but also found in red blood cells, heart cells, muscle tissue and other organs, such as the pancreas and kidneys. An AST test is often performed along with an alanine aminotransferase (ALT) test. Both are enzymes found in the liver that become elevated in the blood when the liver is damaged.

 

AST is usually done as part of a group of liver function tests called a liver panel. It’s often ordered with a test for alanine aminotransferase (ALT), another liver enzyme. ALT is more accurate than AST at detecting liver disease. Abbkine has newly launched ALT test kit to help scientific research.

TCA Tycle

Fig 1: TCA Tycle

(Picture Source: https://www.mdpi.com/1422-0067/20/2/252)

 

Through year of efforts, Abbkine research scientists have developed a series of assay kits for cell metabolism and that constitutes Abbkine CheKine™group. In which we can see the detection kits of NAD/NADH, NADP/NADPH, superoxide dismutases (SOD), peroxidase (HRP) and glucose, but today we are going to introduce the test of ALT for you.

 

To more specific, the benefits of Abbkine Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit can be concluded as below:

  • Determination of ALT/GPT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial.
  • Detailed sample preparation and result calculation methods are provided.
  • Simple operation steps.
  • Standard and standard curve line are provided.

 

You can view the kit protocol at https://www.abbkine.com/file/booklet/KTB1410-B.pdf .

 

Kit Components:

  • Extraction Buffer
  • Reagent I
  • Reagent II
  • Reagent III
  • Standard

 

Abbkine is aimed at to provide high quality and cost effective reagents to global scientists, this    Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit has been optimized to use and can deduct your experiment cost. For detailed product information, you can refer in below sheet.

 

CAT# Product Name Size and Price
KTB1410 CheKine™ Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit $40/96T

 

If you are interested in Abbkine Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit, feel free to contact service@abbkine.com, our service representative will get back to you shortly!

 

About Abbkine Scientific Co., Ltd.

Abbkine offers services for global scientists in the fields of proteomics and cytology, and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, to promote the development of fields including life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, to help your research career!

 

Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are enzymes found mainly in the liver, but also found in red blood cells, heart cells, muscle tissue and other organs, such as the pancreas and kidneys. An AST test is often performed along with an alanine aminotransferase (ALT) test. Both are enzymes found in the liver that become elevated in the blood when the liver is damaged.

 

AST is usually done as part of a group of liver function tests called a liver panel. It’s often ordered with a test for alanine aminotransferase (ALT), another liver enzyme. ALT is more accurate than AST at detecting liver disease. Abbkine has newly launched ALT test kit to help scientific research.

TCA Tycle

Fig 1: TCA Tycle

(Picture Source: https://www.mdpi.com/1422-0067/20/2/252)

 

Through year of efforts, Abbkine research scientists have developed a series of assay kits for cell metabolism and that constitutes Abbkine CheKine™group. In which we can see the detection kits of NAD/NADH, NADP/NADPH, superoxide dismutases (SOD), peroxidase (HRP) and glucose, but today we are going to introduce the test of ALT for you.

 

To more specific, the benefits of Abbkine Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit can be concluded as below:

  • Determination of ALT/GPT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial.
  • Detailed sample preparation and result calculation methods are provided.
  • Simple operation steps.
  • Standard and standard curve line are provided.

 

You can view the kit protocol at https://www.abbkine.com/file/booklet/KTB1410-B.pdf .

 

Kit Components:

  • Extraction Buffer
  • Reagent I
  • Reagent II
  • Reagent III
  • Standard

 

Abbkine is aimed at to provide high quality and cost effective reagents to global scientists, this    Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit has been optimized to use and can deduct your experiment cost. For detailed product information, you can refer in below sheet.

 

CAT# Product Name Size and Price
KTB1410 CheKine™ Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit $40/96T

 

If you are interested in Abbkine Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit, feel free to contact service@abbkine.com, our service representative will get back to you shortly!

 

About Abbkine Scientific Co., Ltd.

Abbkine offers services for global scientists in the fields of proteomics and cytology, and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, to promote the development of fields including life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, to help your research career!

 

http://www.bioadvisers.com/alanine-aminotransferase/

How much do you know about tissue sections? Abbkine analyzes for you one by one

 

 

Not long ago, Yacoin organized a full-blown experimental study of immunopathology, which aroused the interest of many curious babies. Everyone left messages, and the pathology experiment also included immunohistochemistry, pathology detection and other aspects, and there was still a lot of pathology knowledge that was not shown to everyone. Seeing the questions you have left, the editor will announce them one by one on the Yacoin WeChat platform in the follow-up. In this issue, the editor will start from the shallower to the deeper, starting with the knowledge of tissue slices for everyone.

When it comes to tissue sectioning, you will have many questions. Here is an analysis of your questions one by one:

  1. What is a tissue section?

A type of qie pian slide specimen. A thin slice of animal and plant tissues for optical or electron microscope observation. Due to different requirements, a blade can be used for freehand sectioning, or the tissue block can be embedded in paraffin or collodion or frozen at low temperature and sectioned with a microtome. Cut into 5~10 micron slices for observation by optical microscope. Ultra-thin sections cut with epoxy resin or methacrylic acid embedded tissue blocks, with a thickness of 20-50 nanometers, designed for observation under an electron microscope. The sections of root tips and stems used in general teaching are generally called paraffin sections.

 

 

  1.  What is the initial shape of the tissue specimen of the tissue section?

The size of the tissue block: the ideal volume of the tissue block is 2.0cm×2.0cm×0.3cm, so that the fixative can penetrate into the tissue quickly and evenly. However, the tissue block is ideal according to the different preparation materials and purposes The volume is also different. For example, when making pathological examinations and scientific research sections, the tissue block can be thinned by 0.1~0.2cm, which can shorten the time of fixation, dehydration and transparency. If the thickness of the teaching section is 0.3~0.5cm, the same wax can be used. Block makes more teaching slices.

  1. How to make tissue dehydration transparent?

After the specimen is fixed and washed, the tissue contains a lot of water, and the water in the tissue block must be replaced. This process is called dehydration. Whether it is sliced with paraffin or collodion, the water in the tissue must be removed. Because water-containing tissue is incompatible with embedding materials such as paraffin wax and collodion, the commonly used dehydrating agent is a series of ethanol with different concentrations. .

The dehydration steps are: 80%, 90%, 95%, 100% ethanol of various concentrations for 2 hours, this process can be completed by the automatic control of the dehydrator.

Acetone is also a dehydrating agent with strong dehydration ability, but because of its strong dehydration ability, it has a violent contraction effect on tissues, so it is generally not used when making scientific research and teaching sections. Because ethanol, acetone, etc. are not soluble in paraffin, a solvent replacement process that can dissolve in paraffin is called transparent. Commonly used transparent agents are xylene, chloroform, methyl salicylate, etc.

  1. How to perform wax immersion, embedding, sectioning, and patching?

(1) Trimming wax block: Depending on the size of the tissue, cut off the remaining wax at about 0.1~0.2cm at the edge of the tissue, otherwise the tissue may shrink and become uneven.

(2) Prepare slicing tools: slicing knife, writing brush, ophthalmic tweezers (curved), bleaching temperature controller

(3) Install wax block: install the repaired wax block on a metal or wooden wax holder.

(4) Install the slicing knife: Install the slicing knife on the knife table of the slicing machine, and tighten the fastening screws on the knife table to prevent vibration during slicing and maintain a certain slice thickness.

(5) The thickness of the slice: the thickness regulator of the slicer is engraved with 0~50μm or 0~25μm, and the thickness can be selected arbitrarily. The thickness of the paraffin slice is generally 4~6μm.

(6) Slice

(7) Spreading: Use ophthalmic tweezers to lift the wax tape and gently spread it on the water surface at 40~45°C. Use the tension of the water and the temperature of the water to naturally flatten the slightly wrinkled wax tape.

(8) Patches and baking sheets: After the slices are fully flattened on the constant temperature water surface, the wax slices are pulled to the middle of the slide glass and the remaining water on the slide glass is poured, and placed in a 60-65 ℃ incubator Bake the slices in the oven of the temperature controller for 15 to 30 minutes to remove the paraffin that melts the tissue gap.

The picture shows a set of experimental equipment for paraffin sectioning: wax embedding machine, slicer, and spreader.

The picture shows the standard operation method of paraffin section。

  1. How to improve the level of tissue sectioning?

1) Pathology instrument is a necessary condition: choosing the right slicer is the primary factor of the experiment;

2) The quality of the sample to be cut is a key factor: the quality of the sample depends on the accuracy of the pre-processing work, including fixation, dehydration, embedding, etc.;

3) Model matching: suitable steel knife or disposable blade; carefully consider the sample type, size and thickness when slicing, taking into account the convenience and comfort of the operator;

4) The operator’s rich experience is essential: the operator must understand the working principle of the slicer and fine-tune the machine to achieve the best results; be able to deal with common failures and eliminate some potential influencing factors;

5) Elimination of common faults: such as the angle of the slicer blade, the temperature and hardness of the embedding block, whether there are notches or impurities attached to the blade, etc.

This issue mainly talks about the knowledge of tissue sectioning. Time is limited, so I have to say goodbye to everyone. At the same time in the next issue, there will be some tips on pathology experiments. Welcome everyone to leave a message and ask questions.

You are welcome to pay more attention to Abbkine’s official account, and there will be more scientific research surprises waiting for you. At the same time, in order to provide readers with a platform for learning biological knowledge, Abbkine’s technical experts have worked tirelessly to sort out the dry goods of a variety of high-end biological experiments, which will be published in the WeChat public account in the later period. Please continue to pay attention to Abbkine for readers who love science. No public.

Abbkine focuses on the fields of proteinology and cytology, and is committed to the innovation and research and development of various antibodies, proteins, analytical reagents and kits, in order to become a key promoter in the development of life science research, drug development and other fields. We provide you with the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary and secondary antibodies for immunological experiments; the favorite products of cell research users, from Dyes and kits for detecting cell status, organelle extraction kits, cell substructure staining and tracking and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help your research career !

 

 

Not long ago, Yacoin organized a full-blown experimental study of immunopathology, which aroused the interest of many curious babies. Everyone left messages, and the pathology experiment also included immunohistochemistry, pathology detection and other aspects, and there was still a lot of pathology knowledge that was not shown to everyone. Seeing the questions you have left, the editor will announce them one by one on the Yacoin WeChat platform in the follow-up. In this issue, the editor will start from the shallower to the deeper, starting with the knowledge of tissue slices for everyone.

When it comes to tissue sectioning, you will have many questions. Here is an analysis of your questions one by one:

  1. What is a tissue section?

A type of qie pian slide specimen. A thin slice of animal and plant tissues for optical or electron microscope observation. Due to different requirements, a blade can be used for freehand sectioning, or the tissue block can be embedded in paraffin or collodion or frozen at low temperature and sectioned with a microtome. Cut into 5~10 micron slices for observation by optical microscope. Ultra-thin sections cut with epoxy resin or methacrylic acid embedded tissue blocks, with a thickness of 20-50 nanometers, designed for observation under an electron microscope. The sections of root tips and stems used in general teaching are generally called paraffin sections.

 

 

  1.  What is the initial shape of the tissue specimen of the tissue section?

The size of the tissue block: the ideal volume of the tissue block is 2.0cm×2.0cm×0.3cm, so that the fixative can penetrate into the tissue quickly and evenly. However, the tissue block is ideal according to the different preparation materials and purposes The volume is also different. For example, when making pathological examinations and scientific research sections, the tissue block can be thinned by 0.1~0.2cm, which can shorten the time of fixation, dehydration and transparency. If the thickness of the teaching section is 0.3~0.5cm, the same wax can be used. Block makes more teaching slices.

  1. How to make tissue dehydration transparent?

After the specimen is fixed and washed, the tissue contains a lot of water, and the water in the tissue block must be replaced. This process is called dehydration. Whether it is sliced with paraffin or collodion, the water in the tissue must be removed. Because water-containing tissue is incompatible with embedding materials such as paraffin wax and collodion, the commonly used dehydrating agent is a series of ethanol with different concentrations. .

The dehydration steps are: 80%, 90%, 95%, 100% ethanol of various concentrations for 2 hours, this process can be completed by the automatic control of the dehydrator.

Acetone is also a dehydrating agent with strong dehydration ability, but because of its strong dehydration ability, it has a violent contraction effect on tissues, so it is generally not used when making scientific research and teaching sections. Because ethanol, acetone, etc. are not soluble in paraffin, a solvent replacement process that can dissolve in paraffin is called transparent. Commonly used transparent agents are xylene, chloroform, methyl salicylate, etc.

  1. How to perform wax immersion, embedding, sectioning, and patching?

(1) Trimming wax block: Depending on the size of the tissue, cut off the remaining wax at about 0.1~0.2cm at the edge of the tissue, otherwise the tissue may shrink and become uneven.

(2) Prepare slicing tools: slicing knife, writing brush, ophthalmic tweezers (curved), bleaching temperature controller

(3) Install wax block: install the repaired wax block on a metal or wooden wax holder.

(4) Install the slicing knife: Install the slicing knife on the knife table of the slicing machine, and tighten the fastening screws on the knife table to prevent vibration during slicing and maintain a certain slice thickness.

(5) The thickness of the slice: the thickness regulator of the slicer is engraved with 0~50μm or 0~25μm, and the thickness can be selected arbitrarily. The thickness of the paraffin slice is generally 4~6μm.

(6) Slice

(7) Spreading: Use ophthalmic tweezers to lift the wax tape and gently spread it on the water surface at 40~45°C. Use the tension of the water and the temperature of the water to naturally flatten the slightly wrinkled wax tape.

(8) Patches and baking sheets: After the slices are fully flattened on the constant temperature water surface, the wax slices are pulled to the middle of the slide glass and the remaining water on the slide glass is poured, and placed in a 60-65 ℃ incubator Bake the slices in the oven of the temperature controller for 15 to 30 minutes to remove the paraffin that melts the tissue gap.

The picture shows a set of experimental equipment for paraffin sectioning: wax embedding machine, slicer, and spreader.

The picture shows the standard operation method of paraffin section。

  1. How to improve the level of tissue sectioning?

1) Pathology instrument is a necessary condition: choosing the right slicer is the primary factor of the experiment;

2) The quality of the sample to be cut is a key factor: the quality of the sample depends on the accuracy of the pre-processing work, including fixation, dehydration, embedding, etc.;

3) Model matching: suitable steel knife or disposable blade; carefully consider the sample type, size and thickness when slicing, taking into account the convenience and comfort of the operator;

4) The operator’s rich experience is essential: the operator must understand the working principle of the slicer and fine-tune the machine to achieve the best results; be able to deal with common failures and eliminate some potential influencing factors;

5) Elimination of common faults: such as the angle of the slicer blade, the temperature and hardness of the embedding block, whether there are notches or impurities attached to the blade, etc.

This issue mainly talks about the knowledge of tissue sectioning. Time is limited, so I have to say goodbye to everyone. At the same time in the next issue, there will be some tips on pathology experiments. Welcome everyone to leave a message and ask questions.

You are welcome to pay more attention to Abbkine’s official account, and there will be more scientific research surprises waiting for you. At the same time, in order to provide readers with a platform for learning biological knowledge, Abbkine’s technical experts have worked tirelessly to sort out the dry goods of a variety of high-end biological experiments, which will be published in the WeChat public account in the later period. Please continue to pay attention to Abbkine for readers who love science. No public.

Abbkine focuses on the fields of proteinology and cytology, and is committed to the innovation and research and development of various antibodies, proteins, analytical reagents and kits, in order to become a key promoter in the development of life science research, drug development and other fields. We provide you with the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary and secondary antibodies for immunological experiments; the favorite products of cell research users, from Dyes and kits for detecting cell status, organelle extraction kits, cell substructure staining and tracking and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help your research career !

http://www.bioadvisers.com/tissue-section/